Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,547,935	A→G	V54A (GTA→GCA)	adhP ←	alcohol dehydrogenase AdhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,547,935	0	A	G	100.0%	129.5 / NA	38	V54A (GTA→GCA)	adhP	alcohol dehydrogenase AdhP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/19);  total (19/19)

CCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGGC  >  NZ_CP009273/1547849‑1548017                                                                                       |                                                                                   ccacgcTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTgcgc                                                                                 >  1:144796/1‑90 (MQ=255)      cTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGtt                                                                            >  1:933294/1‑90 (MQ=255)             gATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTgcgc                                                                                 <  1:10375/78‑1 (MQ=255)             gATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTgcgc                                                                                 >  2:10375/1‑78 (MQ=255)             gATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCAc                                                                     <  1:664631/90‑1 (MQ=255)             gATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGG‑TTTGTCAcc                                                                    >  2:1240271/1‑90 (MQ=255)             gATCG‑CTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCAcc                                                                    >  1:570900/1‑90 (MQ=255)                          tAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTACATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCAtt                                                        >  1:517976/1‑90 (MQ=255)                                ggTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAAc                                                  >  2:130593/1‑90 (MQ=255)                                ggTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAAc                                                  >  1:587672/1‑90 (MQ=255)                                ggTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAAc                                                  >  1:825344/1‑90 (MQ=255)                                  tGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACAt                                                <  2:69333/90‑1 (MQ=255)                                   gACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATg                                               >  1:620973/1‑90 (MQ=255)                                    acacCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGa                                              <  2:620973/90‑1 (MQ=255)                                         tGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                                         <  2:825344/90‑1 (MQ=255)                                         tGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                                         <  2:130474/90‑1 (MQ=255)                                         tGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                                         <  1:453570/90‑1 (MQ=255)                                         tGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                                         <  1:991591/90‑1 (MQ=255)                                           gACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCgg                                       <  1:116749/90‑1 (MQ=255)                                             cccACTTCTGCGTCCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTa                                     <  1:1206755/90‑1 (MQ=255)                                             cccACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGG‑TTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTAt                                    <  1:1240271/90‑1 (MQ=255)                                                aCTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGa                                  >  1:446534/1‑90 (MQ=255)                                                   tCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACAt                               <  2:933294/90‑1 (MQ=255)                                                        caccacACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATAcacc                          <  1:201943/90‑1 (MQ=255)                                                        caccacACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATAcacc                          <  2:41827/90‑1 (MQ=255)                                                            acacCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCacaa                      <  2:446534/90‑1 (MQ=255)                                                                 gATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTc                 <  1:913333/90‑1 (MQ=255)                                                                 gATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTc                 >  2:913333/1‑90 (MQ=255)                                                                 gATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTc                 <  2:306913/90‑1 (MQ=255)                                                                 gATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTc                 <  2:533151/90‑1 (MQ=255)                                                                  aTGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTaa                                                   <  1:163897/55‑1 (MQ=255)                                                                  aTGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTaa                                                   >  2:163897/1‑55 (MQ=255)                                                                    gCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCAt              >  1:968065/1‑90 (MQ=255)                                                                        tCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTc          >  1:1027524/1‑90 (MQ=255)                                                                              cccAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTATCATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAgg    >  2:347786/1‑90 (MQ=255)                                                                               ccAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAggg   >  1:717852/1‑90 (MQ=255)                                                                               ccAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAggg   >  2:941821/1‑90 (MQ=255)                                                                                cAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGa                                              >  2:685646/1‑46 (MQ=255)                                                                                cAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGa                                              <  1:685646/46‑1 (MQ=255)                                                                                cAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGGc  >  2:57398/1‑90 (MQ=255)                                                                                cAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGGc  >  1:34309/1‑90 (MQ=255)                                                                                       |                                                                                   CCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGGC  >  NZ_CP009273/1547849‑1548017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑