| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,558,439 | A→G | Y518H (TAT→CAT) | dosP ← | oxygen‑sensing cyclic‑di‑GMP phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,558,439 | 0 | A | G | 97.7% | 144.3 / ‑3.6 | 44 | Y518H (TAT→CAT) | dosP | oxygen‑sensing cyclic‑di‑GMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (24/19); total (24/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.89e-01 | |||||||||||
TGCGCCTAAAACCAAACGCTCTTTTACCA‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGTAAG > NZ_CP009273/1558353‑1558520 | tGCGCCTAAACCCAAACTTTCTTTTCCCC‑TTTTGTTAATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATc < 2:1152608/90‑1 (MQ=255)tGCGCCTAAAACCAAACGCTCTTTTACCA‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATc < 2:344233/90‑1 (MQ=255)tGCGCCAAAACCAAAACGCTTTTTTACCT‑TTTCGTTCATCGCGGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATc < 1:71774/90‑1 (MQ=255)tGCGCCAAAAACCAAACGCTTTTTTCCCT‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATc < 1:303950/90‑1 (MQ=255)tGCGCAAAAAACCAACCGTTCTTTTCCCT‑TTTTTTTCATCGCGGGGTTGAAGACCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATc < 1:769946/90‑1 (MQ=255) gcgcCTAAAACCAAACGCTCTTTTACCA‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATcc > 2:38352/1‑90 (MQ=255) cgcCAAAACCCAACCGCTTTTTTCCCT‑TTTCTTTCATCGCGGGGTTGAAGACCTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCca < 1:1001260/90‑1 (MQ=255) gcCTAAACCCAACCGCTTTTTTCCCA‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcat < 1:249313/90‑1 (MQ=255) ccTAAAACCAACCGCTCTTTTACCT‑TTTCTTTCATCGCGGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcatt < 2:643881/90‑1 (MQ=255) tAAAACCAAACGCTCTTTTACCA‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattgc > 1:981679/1‑90 (MQ=255) aaaCGCTCTTTTACCA‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGa > 1:643881/1‑90 (MQ=255) aaaCGCTCTTTTACCA‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGa > 2:794877/1‑90 (MQ=255) aaaCGCTCTTTTACCA‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGa > 1:295346/1‑90 (MQ=255) aaCGCTCTTTTACCA‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAg > 1:927840/1‑90 (MQ=255) aCGCTTTTTTCCCT‑TTTCTTTCATCGCGGGGCTGAAAAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGc < 1:548150/90‑1 (MQ=255) aCGCTTTTTTCCCT‑TTTCTTTCACCCCCGGGCTAAAAAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGc < 1:562607/90‑1 (MQ=255) aCGCTTTTTTACCT‑TTTCTTTCATCCCCGGGCTGAAGATCTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGc < 2:725980/90‑1 (MQ=255) tctTTTACCA‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTg > 2:17493/1‑90 (MQ=255) ctttttTCCATTTTCTTTCACCGCGGGGTTAAAAACCTCCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGt < 1:892894/88‑1 (MQ=255) a‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGc > 1:945044/1‑77 (MQ=255) a‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGc < 2:945044/77‑1 (MQ=255) a‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAg > 1:822152/1‑90 (MQ=255) a‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAg > 1:92702/1‑90 (MQ=255) ttCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCCGTGGAGAGCAAGTa > 2:1111646/1‑90 (MQ=255) ttCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTa > 1:542693/1‑90 (MQ=255) ttCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTa > 2:1223653/1‑90 (MQ=255) ttCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTa > 1:604844/1‑90 (MQ=255) tCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgc < 2:654277/90‑1 (MQ=255) cATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGGGAGCAGTGGAGAGCAAGTAATcgcg > 2:641094/1‑90 (MQ=255) gggCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTa > 1:334220/1‑90 (MQ=255) gtGAAGAACTCCCAGCCGTTCCCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTAccc < 1:418569/89‑1 (MQ=255) cTGAAGACCGCCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTAccc < 1:702797/90‑1 (MQ=255) gaagaaCTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAg > 1:887524/1‑90 (MQ=255) cTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGc < 1:657231/51‑1 (MQ=255) cTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGc > 2:657231/1‑51 (MQ=255) cTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTa > 2:1167433/1‑90 (MQ=255) gCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGc > 1:816294/1‑90 (MQ=255) gCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGc > 1:346233/1‑90 (MQ=255) cAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTg < 2:1171658/90‑1 (MQ=255) cAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTg < 1:317224/90‑1 (MQ=255) tACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCaa < 2:960736/90‑1 (MQ=255) aCCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAAt > 1:844070/1‑90 (MQ=255) cATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCa > 1:540047/1‑90 (MQ=255) gCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGTAAg > 1:995105/1‑90 (MQ=255) | TGCGCCTAAAACCAAACGCTCTTTTACCA‑TTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGTAAG > NZ_CP009273/1558353‑1558520 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |