Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	139,618	A→G	E118G (GAA→GGA)	yadG →	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	139,618	0	A	G	100.0%	196.0 / NA	57	E118G (GAA→GGA)	yadG	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/20);  total (37/20)

TCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAA  >  NZ_CP009273/139531‑139701                                                                                        |                                                                                    tCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGgaa                                                                                   <  2:506468/90‑1 (MQ=255)   aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                                 <  1:637014/90‑1 (MQ=255)        gTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCt                                                                            >  1:99323/1‑90 (MQ=255)                  gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTaa                                                                         <  1:527/83‑1 (MQ=255)                  gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTaa                                                                         >  2:527/1‑83 (MQ=255)                  gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc                                                                  >  1:1104894/1‑90 (MQ=255)                  gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc                                                                  >  2:1141597/1‑90 (MQ=255)                  gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc                                                                  >  1:1212525/1‑90 (MQ=255)                  gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc                                                                  >  2:346357/1‑90 (MQ=255)                  gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc                                                                  >  2:316903/1‑90 (MQ=255)                  gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc                                                                  >  2:1239884/1‑90 (MQ=255)                   tGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCg                                                                 <  1:529980/90‑1 (MQ=255)                    gcagcaAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTTTCTTAAACAACTCGa                                                                <  1:750949/90‑1 (MQ=255)                    gcagcaAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGa                                                                <  1:668061/90‑1 (MQ=255)                            ttGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATggg                                                        >  1:272116/1‑90 (MQ=255)                            ttGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATggg                                                        >  1:121580/1‑90 (MQ=255)                              gtggtgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGa                                                      <  1:1172089/90‑1 (MQ=255)                                ggtgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGaaa                                                    <  1:446965/90‑1 (MQ=255)                                ggtgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGaaa                                                    <  1:622156/90‑1 (MQ=255)                                   gAATCAGGCAGGGTACTACGGCGTGGAGCGCCCACAAGCGTACATCCGGATCGGAAAGCATCTTAAACAACTCGAACAATGGGGAAAAcc                                                 >  2:622788/1‑89 (MQ=255)                                   gAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACg                                                 >  1:727577/1‑90 (MQ=255)                                      tCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaa                                              <  2:1212525/90‑1 (MQ=255)                                        aggcaggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaacg                                            >  2:21076/1‑90 (MQ=255)                                             gggTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt                                       <  2:121580/90‑1 (MQ=255)                                               gTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTgc                                     <  1:1043432/90‑1 (MQ=255)                                                   tacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCg                                                                 <  1:664194/58‑1 (MQ=255)                                                   tacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCg                                                                 >  2:664194/1‑58 (MQ=255)                                                    acGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTa                                <  1:299304/90‑1 (MQ=255)                                                       gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                             >  2:1234239/1‑90 (MQ=255)                                                       gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                             >  2:1087625/1‑90 (MQ=255)                                                       gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                             >  1:322469/1‑90 (MQ=255)                                                       gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                             >  1:325220/1‑90 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTGTCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                           >  1:193233/1‑90 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATc                                                              <  2:1133522/55‑1 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATc                                                              >  1:1133522/1‑55 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                           >  2:398254/1‑90 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                           >  2:786501/1‑90 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                           >  2:774283/1‑90 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                           >  2:549300/1‑90 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                           >  1:980785/1‑90 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                           >  2:269592/1‑90 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                           >  2:24522/1‑90 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                           >  2:154359/1‑90 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                           >  2:129972/1‑90 (MQ=255)                                                         gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                           >  1:569691/1‑90 (MQ=255)                                                            gAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCt                        <  2:811122/90‑1 (MQ=255)                                                               cgcAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGc                     <  1:1234239/90‑1 (MQ=255)                                                                gcAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAaac                                                                       <  2:1125340/39‑1 (MQ=39)                                                                gcAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAaac                                                                       >  1:1125340/1‑39 (MQ=39)                                                                gcAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCg                    >  2:90315/1‑90 (MQ=255)                                                                   aagaagCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGa                 >  1:347052/1‑90 (MQ=255)                                                                         cGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgc           <  1:339115/90‑1 (MQ=255)                                                                          gTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGa                 >  1:751972/1‑83 (MQ=255)                                                                          gTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGa                 <  2:751972/83‑1 (MQ=255)                                                                           tACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc         <  2:980785/90‑1 (MQ=255)                                                                             cATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCg       >  2:1198686/1‑90 (MQ=255)                                                                                  gCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTaa  >  2:747249/1‑90 (MQ=255)                                                                                  gCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTaa  >  2:1030232/1‑90 (MQ=255)                                                                                        |                                                                                    TCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAA  >  NZ_CP009273/139531‑139701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑