Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,624,224	T→G	E316A (GAA→GCA)	ydfI ←	mannitol dehydrogenase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,624,224	0	T	G	100.0%	108.9 / NA	32	E316A (GAA→GCA)	ydfI	mannitol dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/18);  total (14/18)

GCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACTGCCGTTGAGCATGCGCA  >  NZ_CP009273/1624141‑1624305                                                                                    |                                                                                  gCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATAc                                                                             <  2:622663/90‑1 (MQ=255) gCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATAc                                                                             >  1:150213/1‑90 (MQ=255)  cAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACa                                                                            <  2:52322/90‑1 (MQ=255)     aTCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTc                                                                         <  1:197953/90‑1 (MQ=255)         aCGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCATTa                                                                     <  1:1089210/90‑1 (MQ=255)         aCGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCATTa                                                                     <  1:950065/90‑1 (MQ=255)                     ttCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGatat                                                         <  2:1160510/90‑1 (MQ=255)                         aCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTg                                                     >  2:962351/1‑90 (MQ=255)                          cGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCAtt                                                                      <  2:897735/72‑1 (MQ=255)                          cGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCAtt                                                                      >  1:897735/1‑72 (MQ=255)                            tCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATATCGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCaa                                                  <  1:55198/90‑1 (MQ=255)                              ggCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGa                                                <  1:792197/90‑1 (MQ=255)                               gcgcTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGAt                                               <  2:1213197/90‑1 (MQ=255)                                cgcTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATa                                              <  1:1069705/90‑1 (MQ=255)                                        ccTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGAt                                      <  1:199713/90‑1 (MQ=255)                                         cTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATa                                     >  2:763759/1‑90 (MQ=255)                                         cTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATa                                     >  1:582244/1‑90 (MQ=255)                                              aCATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCa                                <  1:1103193/90‑1 (MQ=255)                                              aCATCAAGCCATACGCCGCATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCa                                <  1:235255/90‑1 (MQ=255)                                                           cgccgcATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACt                   <  1:299207/90‑1 (MQ=255)                                                               gcATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACTGCCg               >  1:925304/1‑90 (MQ=255)                                                               gcATAACGATAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACTGCCg               >  2:464746/1‑90 (MQ=255)                                                                       aTAATGTTCATCTGCCATACAGTCATTAATGTGCTGatat                                                         >  2:137259/1‑40 (MQ=255)                                                                       aTAATGTTCATCTGCCATACAGTCATTAATGTGCTGatat                                                         >  2:420572/1‑40 (MQ=255)                                                                       aTAATGTTCATCTGCCATACAGTCATTAATGTGCTGatat                                                         <  1:137259/40‑1 (MQ=255)                                                                       aTAATGTTCATCTGCCATACAGTCATTAATGTGCTGatat                                                         <  1:420572/40‑1 (MQ=255)                                                                       aTAATGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACTGCCGTTGAGCAt       >  1:703750/1‑90 (MQ=255)                                                                         aaTGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACTGCCGTTGAGCATgc     >  1:318535/1‑90 (MQ=255)                                                                         aaTGTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACTGCCGTTGAGCATgc     >  1:310450/1‑90 (MQ=255)                                                                            gTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACTGCCGTTGAGCATGCGCa  <  2:1053531/90‑1 (MQ=255)                                                                            gTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACTGCCGTTGAGCATGCGCa  >  2:833856/1‑90 (MQ=255)                                                                            gTTCATCTGCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACTGCCGTTGAGCATGCGCa  >  1:295372/1‑90 (MQ=255)                                                                                    |                                                                                  GCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACTGCCGTTGAGCATGCGCA  >  NZ_CP009273/1624141‑1624305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑