Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,662,649	T→G	H58P (CAC→CCC)	mlc ←	DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,662,649	0	T	G	100.0%	89.1 / NA	27	H58P (CAC→CCC)	mlc	DNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/13);  total (14/13)

GTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGC  >  NZ_CP009273/1662564‑1662726                                                                                      |                                                                              gTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGc                                                                           >  1:116447/1‑90 (MQ=255) gTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGc                                                                           >  2:225619/1‑90 (MQ=255) gTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGc                                                                           >  1:1060462/1‑90 (MQ=255) gTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGc                                                                           >  1:470765/1‑90 (MQ=255) gTGCCAAGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCATGCACCAGGGGTGc                                                                           >  1:702059/1‑90 (MQ=255)   gCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCtt                                                                         <  1:1146641/90‑1 (MQ=255)                 tCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACgg                                                           >  2:720938/1‑90 (MQ=255)                 tCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACgg                                                           <  1:720938/90‑1 (MQ=255)                   aaCCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGAc                                                         <  1:764367/90‑1 (MQ=255)                   aaCCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGAc                                                         <  2:982674/90‑1 (MQ=255)                          aGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTa                                                  >  1:288063/1‑90 (MQ=255)                           gCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAg                                                 <  2:288063/90‑1 (MQ=255)                           gCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAg                                                 >  1:1239358/1‑90 (MQ=255)                           gCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAg                                                 <  1:755282/90‑1 (MQ=255)                              ccACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGa                                              >  2:258777/1‑90 (MQ=255)                                aCCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATa                                            <  1:1119920/90‑1 (MQ=255)                                                    ccccGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTgcgc                        <  2:500273/90‑1 (MQ=255)                                                     cccGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGcc                       >  2:1008345/1‑90 (MQ=255)                                                     cccGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGcc                       >  1:665551/1‑90 (MQ=255)                                                           tcttTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACgg                 >  1:273713/1‑90 (MQ=255)                                                           tcttTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACgg                 >  2:941394/1‑90 (MQ=255)                                                              ttGATTTCCAGCTCTTGCCCCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGaa              <  2:779261/90‑1 (MQ=255)                                                                   ttCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATc         <  2:474386/90‑1 (MQ=255)                                                                          tctTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATAcgc  <  2:45569/90‑1 (MQ=255)                                                                          tctTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATAcgc  <  2:470765/90‑1 (MQ=255)                                                                           ctTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATc         >  1:281737/1‑82 (MQ=255)                                                                           ctTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATc         <  2:281737/82‑1 (MQ=255)                                                                                      |                                                                              GTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGC  >  NZ_CP009273/1662564‑1662726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑