Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,673,542	A→G	K287E (AAG→GAG)	ydgH →	DUF1471 family protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,673,542	0	A	G	100.0%	79.3 / NA	27	K287E (AAG→GAG)	ydgH	DUF1471 family protein YdgH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/17);  total (10/17)

ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAA  >  NZ_CP009273/1673458‑1673624                                                                                     |                                                                                   atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                               <  1:156398/90‑1 (MQ=255) atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                               <  2:407606/90‑1 (MQ=255) atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                               <  1:491862/90‑1 (MQ=255)   ggTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAgg                                                                             >  2:698721/1‑90 (MQ=255)     tCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTg                                                                           >  2:179581/1‑90 (MQ=255)      cccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGc                                                                          <  2:751120/90‑1 (MQ=255)      cccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGc                                                                          <  1:1033196/90‑1 (MQ=255)       ccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt                                                                         <  2:592813/90‑1 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                                       <  1:301565/90‑1 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                                       <  2:834148/90‑1 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                                       <  2:359427/90‑1 (MQ=255)              aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt                                                                  >  2:1070945/1‑90 (MQ=255)                   aTCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACcac                                                             <  2:1078312/90‑1 (MQ=255)                           ctctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCg                                                     >  1:1096562/1‑90 (MQ=255)                                gCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGt                                                <  2:606369/90‑1 (MQ=255)                                           aaCATGCCCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGt                                     <  2:93668/90‑1 (MQ=255)                                           aaCATGCCCGAAGTCTCCTATCAGGTTGCGAAACGGGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGt                                     <  2:547772/90‑1 (MQ=255)                                              aTGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGGACGTggg                                  >  1:967476/1‑89 (MQ=255)                                                    gAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAAc                            >  2:478249/1‑90 (MQ=255)                                                         ctcCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGAc                       >  2:69903/1‑90 (MQ=255)                                                         ctcCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGAc                       >  2:228667/1‑90 (MQ=255)                                                         ctcCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGAc                       >  2:1008594/1‑90 (MQ=255)                                                             taccAGGTGGCAAACCGCGCCGGGGAAAAAGGTGCAAATTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc                   <  1:985031/87‑1 (MQ=255)                                                             tATCGGGTTGCAAACCGTGCCGGGGAGAAAGGTGCTAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc                   <  1:321010/90‑1 (MQ=255)                                                                      gCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGGAATAACCTGACCGTCAGCGCAGAt          >  2:321010/1‑90 (MQ=255)                                                                       cGAAACGTGCCGCGGAGAAAGGTGCTAGGTTTTCCCCCATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc         <  2:961288/90‑1 (MQ=255)                                                                              tGCCGCGGAAAAAGGTGCAAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataa  <  1:478249/90‑1 (MQ=255)                                                                                     |                                                                                   ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAA  >  NZ_CP009273/1673458‑1673624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑