Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,717,498	G→C	G374A (GGC→GCC)	ydhK →	FUSC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,717,498	0	G	C	100.0%	151.8 / NA	44	G374A (GGC→GCC)	ydhK	FUSC family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (26/18);  total (26/18)

CACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTC  >  NZ_CP009273/1717411‑1717576                                                                                        |                                                                               cACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGccg                                                                              <  2:651269/90‑1 (MQ=255)    ggACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCAt                                                                           <  1:224937/90‑1 (MQ=255)    ggACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCAt                                                                           <  2:1157475/90‑1 (MQ=255)    ggACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCAt                                                                           <  1:829439/90‑1 (MQ=255)       cATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGa                                                                        >  2:291352/1‑90 (MQ=255)       cATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGa                                                                        >  2:127880/1‑90 (MQ=255)       cATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGa                                                                        >  1:244590/1‑90 (MQ=255)         tCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGt                                                                      >  2:231361/1‑90 (MQ=255)         tCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGt                                                                      >  1:914632/1‑90 (MQ=255)            ggCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTAtt                                                                   <  2:914632/90‑1 (MQ=255)             gCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTg                                                                  >  1:719040/1‑90 (MQ=255)               cTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCAt                                                                           >  1:1151710/1‑79 (MQ=255)               cTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCAt                                                                           <  2:1151710/79‑1 (MQ=255)                      gTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAAt                                                         <  1:101571/90‑1 (MQ=255)                      gTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAAt                                                         >  2:322370/1‑90 (MQ=255)                          tACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGa                                                     <  1:471782/90‑1 (MQ=255)                          tACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGa                                                     >  2:471782/1‑90 (MQ=255)                                tAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCgg                                               >  1:710781/1‑90 (MQ=255)                                tAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCgg                                               >  1:275002/1‑90 (MQ=255)                                      cGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCaa                                         <  2:150245/90‑1 (MQ=255)                                        aaGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGcc                                                 >  2:830888/1‑80 (MQ=255)                                        aaGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGcc                                                 <  1:830888/80‑1 (MQ=255)                                                tgGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACg                               <  1:1181097/90‑1 (MQ=255)                                                  gAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCt                             >  1:365632/1‑90 (MQ=255)                                                  gAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCt                             >  1:682974/1‑90 (MQ=255)                                                    gCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTgg                           >  1:846782/1‑90 (MQ=255)                                                     cGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTAt                                                                    <  2:525091/48‑1 (MQ=255)                                                     cGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTAt                                                                    >  1:525091/1‑48 (MQ=255)                                                      gggCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGgca                         >  2:217471/1‑90 (MQ=255)                                                       ggCTGCGTACATTTTGTACGTTGATGATGATTGCCGCAt                                                                           <  2:413787/39‑1 (MQ=39)                                                       ggCTGCGTACATTTTGTACGTTGATGATGATTGCCGCAt                                                                           >  1:413787/1‑39 (MQ=39)                                                       ggCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGgcag                        <  2:1135507/90‑1 (MQ=255)                                                         cTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGgcagca                      >  2:1181001/1‑90 (MQ=255)                                                         cTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGgcagca                      >  1:1046335/1‑90 (MQ=255)                                                                 aTTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTg              <  1:231361/90‑1 (MQ=255)                                                                 aTTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTg              >  2:543135/1‑90 (MQ=255)                                                                 aTTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTg              >  2:569073/1‑90 (MQ=255)                                                                 aTTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTg              <  1:431080/90‑1 (MQ=255)                                                                   tttGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCGGCAATTAGCTGCg            <  2:846782/90‑1 (MQ=255)                                                                     tGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGta          >  1:615385/1‑90 (MQ=255)                                                                      gTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGtac         <  2:306236/90‑1 (MQ=255)                                                                      gTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGtac         <  1:314951/90‑1 (MQ=255)                                                                          gTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGtactcta     >  2:450434/1‑90 (MQ=255)                                                                            tgatgatgaTTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGtactctact   >  2:1087060/1‑90 (MQ=255)                                                                             gatgatgatTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGtactctactc  >  1:256810/1‑90 (MQ=255)                                                                                        |                                                                               CACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTC  >  NZ_CP009273/1717411‑1717576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑