Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,782,276	T→G	V192G (GTG→GGG)	ppsR →	posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,782,276	0	T	G	100.0%	65.9 / NA	20	V192G (GTG→GGG)	ppsR	posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/4);  total (16/4)

CCCCACCAGTCTGTATCTGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGTGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTGGCGGCGATTCGCGAG  >  NZ_CP009273/1782193‑1782358                                                                                    |                                                                                   ccccACCAGTCTGTATCTGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTAcc                                                                              >  2:758939/1‑90 (MQ=255)   ccaccaGTCTGTATCTGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCcg                                                                            >  1:55498/1‑90 (MQ=255)   ccaccaGTCTGTATCTGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCcg                                                                            >  2:359386/1‑90 (MQ=255)         gTCTGTATCTGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGc                                                                      >  2:725514/1‑90 (MQ=255)           cTGTATCTGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTc                                                                    >  1:541499/1‑90 (MQ=255)             gTATCTGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCaa                                                                  >  1:814665/1‑90 (MQ=255)                tCTGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAAcc                                                               <  2:710910/90‑1 (MQ=255)                   ggCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCt                                                            >  2:401684/1‑90 (MQ=255)                    gCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCtt                                                           <  1:456426/90‑1 (MQ=255)                       aTGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAg                                                        >  1:435137/1‑90 (MQ=255)                       aTGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAg                                                        >  1:701824/1‑90 (MQ=255)                                 gTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGt                                              >  1:1017602/1‑90 (MQ=255)                                  tATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGtt                                             <  2:701824/90‑1 (MQ=255)                                     ccGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCgg                                          >  2:262920/1‑90 (MQ=255)                                                        tttATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGaa                       <  1:483410/90‑1 (MQ=255)                                                           aTTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGt                    >  2:1139999/1‑90 (MQ=255)                                                              gCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTg                 >  2:120913/1‑90 (MQ=255)                                                              gCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTg                 >  1:143948/1‑90 (MQ=255)                                                                        tGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTGGCGGCGATTc       >  2:331099/1‑90 (MQ=255)                                                                             aaTCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTGGCGGCGATTCGCGAg  >  1:456280/1‑90 (MQ=255)                                                                                    |                                                                                   CCCCACCAGTCTGTATCTGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGTGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTGGCGGCGATTCGCGAG  >  NZ_CP009273/1782193‑1782358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑