Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	264,689	(A)5→4	intergenic (‑18/+311)	ykfA ← / ← perR	GTPase family protein/LysR substrate‑binding domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	264,685	0	A	.	100.0%	29.7 / NA	8	intergenic (‑14/+315)	ykfA/perR	GTPase family protein/LysR substrate‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (4/4);  total (4/4)

TGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTT  >  NZ_CP009273/264606‑264766                                                                                |                                                                                  tGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATaaa                                                                        >  2:315271/1‑90 (MQ=255)         aTTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTa                                                                <  1:315271/90‑1 (MQ=255)                                       aCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTa                                  <  2:260776/90‑1 (MQ=255)                                                    ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat                     >  1:165567/1‑90 (MQ=255)                                                    ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat                     <  1:55787/90‑1 (MQ=255)                                                     tCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgatg                    <  2:85550/90‑1 (MQ=255)                                                             attCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGatatatat            >  2:117922/1‑90 (MQ=255)                                                                       actCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGAttt  >  2:163225/1‑90 (MQ=255)                                                                                |                                                                                  TGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTT  >  NZ_CP009273/264606‑264766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑