| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,816,454 | 0 | A | C | 53.3% | 4.4 / 14.6 | 15 | G20G (GGT→GGG) | osmE | osmotically‑inducible lipoprotein OsmE |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base C (0/8); total (3/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCGT > NZ_CP009273/1816366‑1816538 | gtgCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAAc > 1:69630/1‑90 (MQ=255) cTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAg < 1:56866/90‑1 (MQ=255) tttGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCAAAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGc < 1:109166/90‑1 (MQ=255) tGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACACCCCGCCAGCATGGTTAATACCGCCGCTGCAc < 2:50290/90‑1 (MQ=255) tGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCAc < 1:91922/90‑1 (MQ=255) taaaaaCTGGTCTTTGGTACGATCATAAGCCGTACACCCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCtt < 1:106490/87‑1 (MQ=255) tACAACCTGGTCTTTGGCACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCtt < 1:14541/90‑1 (MQ=255) tttttGTTACAATAAAAAGCCGTACACCCCGCCGGCATGTTTAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTc < 1:278981/88‑1 (MQ=255) tAAGCCGTACACCCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGt < 1:117386/78‑1 (MQ=255) tAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGt > 2:117386/1‑78 (MQ=255) cccGAACACCCCCCCAGCATGTTTAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAg < 2:135062/89‑1 (MQ=255) ccttACACCCCGCCAGCATGTTTAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGc < 1:121230/87‑1 (MQ=255) ccttACACCCCGCCAGCATGGTTAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGc < 1:298152/87‑1 (MQ=255) ccGTACACCCCGCCAGCATGTTTAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGc < 1:30540/90‑1 (MQ=255) atACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCgt > 1:347149/2‑90 (MQ=255) | GTGCAACCTGCGCCCGGCTCATGCCTTTTTTGACGTCTTTCACCACAGGCTGTACAAACTGGTCTTTGGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCGT > NZ_CP009273/1816366‑1816538 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |