Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 221,335 T→C noncoding (1078/1542 nt) BW25113_RS01010 → 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273221,3350TC100.0% 53.6 / NA 18noncoding (1078/1542 nt)BW25113_RS0101016S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/10);  total (8/10)

ATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCC  >  NZ_CP009273/221253‑221419
                                                                                  |                                                                                    
aTCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGt                                                                               <  1:276176/90‑1 (MQ=255)
 tCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGtt                                                                              >  1:145407/1‑90 (MQ=255)
 tCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGtt                                                                              >  2:77307/1‑90 (MQ=255)
  ccACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGt                                                                               >  1:358624/1‑88 (MQ=255)
  ccACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGt                                                                               <  2:358624/88‑1 (MQ=255)
  ccACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTg                                                                             >  1:184469/1‑90 (MQ=255)
  ccACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTg                                                                             >  2:146549/1‑90 (MQ=255)
                 gagaTGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCaa                                                              <  1:77307/90‑1 (MQ=255)
                   gaTGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACg                                                            <  1:202105/90‑1 (MQ=255)
                   gaTGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCGGCATGGCTGTCGTCAGGTCGTGTTGCGAGATGTTGGGTTAAGTCCCGCAACg                                                            >  1:318168/1‑90 (MQ=14)
                        aTTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAgcgc                                                       <  2:144957/90‑1 (MQ=255)
                         ttGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCa                                                      <  2:44202/90‑1 (MQ=255)
                         ttGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCa                                                      <  1:135288/90‑1 (MQ=255)
                                                           aTGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCggg                    <  1:146549/90‑1 (MQ=25)
                                                           aTGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCggg                    <  2:144043/90‑1 (MQ=25)
                                                           aTGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCggg                    <  2:73261/90‑1 (MQ=25)
                                                              gCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAAc                 >  1:313371/1‑90 (MQ=18)
                                                                 gtcgtcAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCa              >  2:325768/1‑90 (MQ=18)
                                                                             tgttgcGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGcc  <  2:149520/90‑1 (MQ=18)
                                                                                  |                                                                                    
ATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCC  >  NZ_CP009273/221253‑221419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: