Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,012,918	A→G	D351G (GAC→GGC)	fliK →	flagellar hook‑length control protein FliK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,012,918	0	A	G	100.0%	16.4 / NA	6	D351G (GAC→GGC)	fliK	flagellar hook‑length control protein FliK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/2);  total (4/2)

GAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAA  >  NZ_CP009273/2012833‑2012994                                                                                      |                                                                             gAAAGCTTTAGTGGTCAGCAGGAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAggcgac                                                                          >  2:66590/1‑90 (MQ=255)             ggTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCg                                                              >  1:171094/1‑90 (MQ=255)                   cagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTccc                                                        <  1:326405/90‑1 (MQ=255)                               tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGTCTCTTTACaa                                            <  2:246156/90‑1 (MQ=255)                                                  aaCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACa                         >  2:256245/1‑90 (MQ=255)                                                                  tGAACCTCTGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt         >  2:40243/1‑90 (MQ=255)                                                                         ctGGCGGGGGAAGGCGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTaa  >  2:203440/1‑90 (MQ=255)                                                                                      |                                                                             GAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAA  >  NZ_CP009273/2012833‑2012994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑