Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,122,267	A→C	S35A (TCG→GCG)	wcaF ←	colanic acid biosynthesis acetyltransferase WcaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,122,267	0	A	C	100.0%	31.3 / NA	10	S35A (TCG→GCG)	wcaF	colanic acid biosynthesis acetyltransferase WcaF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (1/9);  total (1/9)

GGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCA  >  NZ_CP009273/2122185‑2122308                                                                                   |                                          ggacgAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCa                                    <  2:59151/90‑1 (MQ=255)    cgAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAat                                 <  2:343490/90‑1 (MQ=255)        tAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATAttg                             <  1:13040/90‑1 (MQ=255)        tAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATAttg                             <  2:141914/90‑1 (MQ=255)        tAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATAttg                             <  2:33243/90‑1 (MQ=255)        tAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATAttg                             <  2:53526/90‑1 (MQ=255)             aCGTTTTTTCCTATTCTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGcc                        <  1:17216/90‑1 (MQ=255)                       cTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGCCTGTACTGccc              <  1:336387/90‑1 (MQ=255)                       cTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGCCTGTACTGccc              <  2:98378/90‑1 (MQ=255)                                   cGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGCCCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCa  >  2:236275/1‑90 (MQ=255)                                                                                   |                                          GGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCA  >  NZ_CP009273/2122185‑2122308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑