Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,140,422	G→C	G200G (GGC→GGG)	alkA ←	DNA‑3‑methyladenine glycosylase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,140,422	0	G	C	100.0%	24.3 / NA	8	G200G (GGC→GGG)	alkA	DNA‑3‑methyladenine glycosylase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (2/6);  total (2/6)

CGCAAAGCAAAATAATTCGCCGTCCAGCGCCCGATACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCA  >  NZ_CP009273/2140345‑2140501                                                                              |                                                                                cGCAAAGCAAAATAATTCGCCGTCCAGCGCCCGATACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCCCCCGGTATTGTc                                                                     <  2:10512/90‑1 (MQ=255)   caaagcaaaATAATTCGCCGTCCAGCGCCCGATACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCCCCCGGTATTGTCAt                                                                   <  1:355780/90‑1 (MQ=255)     aagcaaaATAATTCGCCGTCCAGCGCCCGATACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCCCCCGGTATTGTCATTg                                                                 >  1:94128/1‑90 (MQ=255)         aaaaTAATTCGCCGTCCAGCGCCCGATACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCCCCCGGTATTGTCATTGGTaa                                                             <  1:328565/90‑1 (MQ=255)               atTCGCCGTCCAGCGCCCGATACCCGGAAAAGTTTGCAGCGTTTTCATCGCATGCTCCACATCCCCCGGTATTGTCATTGGTAAGGTGcc                                                       >  2:208014/1‑90 (MQ=255)                                              gTTTGCAGCGTTTTCATCGCCTGCTCCACATCCCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAgc                        <  2:56161/90‑1 (MQ=255)                                                                    gCTCCACATCCCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCa  <  1:86679/90‑1 (MQ=255)                                                                    gCTCCACATCCCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCa  <  2:158028/90‑1 (MQ=255)                                                                              |                                                                                CGCAAAGCAAAATAATTCGCCGTCCAGCGCCCGATACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCA  >  NZ_CP009273/2140345‑2140501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑