| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,439,101 | T→C | K255E (AAG→GAG) | mepA ← | penicillin‑insensitive murein endopeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,439,101 | 0 | T | C | 100.0% | 35.3 / NA | 11 | K255E (AAG→GAG) | mepA | penicillin‑insensitive murein endopeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (6/5); total (6/5) | |||||||||||
TAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGGTAAAGGT > NZ_CP009273/2439015‑2439186 | tAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTctcctc < 1:6691/90‑1 (MQ=255) aaaCAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCa < 1:102210/90‑1 (MQ=255) ggCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCtt > 1:37415/1‑90 (MQ=255) ggCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCtt > 2:39825/1‑90 (MQ=255) tCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGtt > 1:162456/1‑90 (MQ=255) cGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCaa > 1:302253/1‑90 (MQ=255) aGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGc < 2:274162/90‑1 (MQ=255) gcCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCt < 1:194550/90‑1 (MQ=255) gcCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCt < 1:194551/90‑1 (MQ=255) gcCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCt > 2:37675/1‑90 (MQ=255) gAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATg < 2:162456/90‑1 (MQ=255) ggTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCggg > 2:180098/1‑89 (MQ=255) ttctcctCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGGTAAAGGt < 1:4181/90‑1 (MQ=255) | TAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGGTAAAGGT > NZ_CP009273/2439015‑2439186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |