Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	297,509	G→C	G30G (GGC→GGG)	paoB ←	aldehyde oxidoreductase FAD‑binding subunit PaoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	297,509	0	G	C	100.0%	51.9 / NA	15	G30G (GGC→GGG)	paoB	aldehyde oxidoreductase FAD‑binding subunit PaoB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/5);  total (10/5)

ATCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCAT  >  NZ_CP009273/297428‑297582                                                                                  |                                                                          aTCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGGGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGAt                                                                   >  2:288398/1‑90 (MQ=255)  tCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATa                                                                  <  2:48741/90‑1 (MQ=255)                 ttCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGt                                                   >  2:147968/1‑90 (MQ=255)                          aTAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGa                                          >  1:346814/1‑90 (MQ=255)                           tAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAg                                         <  1:142815/90‑1 (MQ=255)                               gtgggtggGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCt                                     >  1:111297/1‑90 (MQ=255)                                 gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTaa                                   >  1:350001/1‑90 (MQ=255)                                 gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTaa                                   >  2:1213/1‑90 (MQ=255)                                 gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTaa                                   >  2:158022/1‑90 (MQ=255)                                 gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTaa                                   >  2:62182/1‑90 (MQ=255)                                   gtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGt                                 >  1:100699/1‑90 (MQ=255)                                   gtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGt                                 >  2:234320/1‑90 (MQ=255)                                         gTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTgccgcc                           <  2:131789/90‑1 (MQ=255)                                                          catcaGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCAc          <  2:58572/90‑1 (MQ=255)                                                                  ccAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCAt  <  1:131004/90‑1 (MQ=255)                                                                                  |                                                                          ATCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCAT  >  NZ_CP009273/297428‑297582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑