Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,536,106	T→C	E261G (GAA→GGA)	cysP ←	thiosulfate/sulfate ABC transporter substrate‑binding protein CysP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,536,106	0	T	C	100.0%	43.7 / NA	13	E261G (GAA→GGA)	cysP	thiosulfate/sulfate ABC transporter substrate‑binding protein CysP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/2);  total (11/2)

ATTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATCACCACTTCAA  >  NZ_CP009273/2536024‑2536190                                                                                   |                                                                                     aTTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTAcc                                                                               >  1:220272/1‑90 (MQ=255)                    tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  2:15771/1‑90 (MQ=255)                    tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  2:306395/1‑90 (MQ=255)                       gTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCa                                                         >  2:161004/1‑90 (MQ=255)                                 tttGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGC‑TTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCAc                                              >  1:62242/1‑90 (MQ=255)                                         tGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAAt                                       >  1:105468/1‑90 (MQ=255)                                         tGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTGTTTATCAACCCACGCCACCGGGAAt                                       >  2:87209/1‑90 (MQ=255)                                          gCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAAtt                                      >  2:274961/1‑90 (MQ=255)                                                      gCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCa                                                                    <  1:139536/48‑1 (MQ=255)                                                      gCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCa                                                                    >  2:139536/1‑48 (MQ=255)                                                      gCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGt                          >  2:64259/1‑90 (MQ=255)                                                         aGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTgg                       >  2:198941/1‑90 (MQ=255)                                                                              cTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATCACCACTTCaa  <  2:110271/90‑1 (MQ=255)                                                                                   |                                                                                     ATTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATCACCACTTCAA  >  NZ_CP009273/2536024‑2536190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑