Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,833,262	T→G	V127G (GTC→GGC)	ascF →	PTS cellobiose/arbutin/salicin transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,833,262	0	T	G	100.0%	68.4 / NA	20	V127G (GTC→GGC)	ascF	PTS cellobiose/arbutin/salicin transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (11/9);  total (11/9)

ACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGTCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGA  >  NZ_CP009273/2833179‑2833339                                                                                    |                                                                              aCGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAAct                                                                         <  2:346265/90‑1 (MQ=255)        aTTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCa                                                                  <  1:223153/90‑1 (MQ=255)         ttGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCCGGCaa                                                                 >  2:335329/1‑90 (MQ=255)              gCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATc                                                            >  2:191673/1‑90 (MQ=255)                  ggATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCg                                                        <  2:335458/90‑1 (MQ=255)                     tCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATg                                                               >  1:211726/1‑80 (MQ=255)                     tCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATg                                                               <  2:211726/80‑1 (MQ=255)                      ccTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGGTCGATGGGCAAACTGCTGGCAATGATCCTCGAGAt                                                    >  2:322845/1‑90 (MQ=255)                       cTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATg                                                   <  2:310927/90‑1 (MQ=255)                                   aTCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTg                                       <  2:106117/90‑1 (MQ=255)                                       gCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACaa                                   >  2:305241/1‑90 (MQ=255)                                       gCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCCCGAGATGAGCGGCGTGCTGACaa                                   >  2:273916/1‑90 (MQ=255)                                            aTGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGa                              >  1:110608/1‑90 (MQ=255)                                            aTGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGa                              >  1:305208/1‑90 (MQ=255)                                            aTGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGa                              >  1:105060/1‑90 (MQ=255)                                                 aCCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGcc                         <  1:245698/90‑1 (MQ=255)                                                 aCCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGcc                         <  2:820/90‑1 (MQ=255)                                                       gATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCGATGTTGCGCGAGGTTAGGGGCGTGGTGACAAAAAGATTGCCGAccct                   >  2:181444/1‑88 (MQ=255)                                                       gATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCtt                   >  2:74476/1‑90 (MQ=255)                                                                        gcggATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGa  <  1:191673/90‑1 (MQ=255)                                                                                    |                                                                              ACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGTCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGA  >  NZ_CP009273/2833179‑2833339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑