Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,347,716	A→G	intergenic (‑307/‑368)	yhcC ← / → gltB	TIGR01212 family radical SAM protein/glutamate synthase large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,347,716	0	A	G	100.0%	39.3 / NA	12	intergenic (‑307/‑368)	yhcC/gltB	TIGR01212 family radical SAM protein/glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/1);  total (11/1)

TCATTACCAATTAAGGCAGTATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGC  >  NZ_CP009273/3347629‑3347799                                                                                        |                                                                                    tCATTACCAATTAAGGCAGTATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGGAt                                                                                   >  1:285566/1‑90 (MQ=255)                  aGTATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGGATTGACTGTTTCTCTAACg                                                                  <  2:266747/90‑1 (MQ=255)                             cTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGGATTGACTGTTTCTCTAACGACTTCCCtttt                                                       >  1:359104/1‑90 (MQ=255)                                                      ggATAAGGGTTAACACACCTTTATGACAGTCAGGGATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTa                              >  2:303780/1‑90 (MQ=255)                                                                tAACACACCTTTATGACAGTCAGGGATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCa                    >  2:210574/1‑90 (MQ=255)                                                                tAACACACCTTTATGACAGTCAGGGATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCa                    >  2:79883/1‑90 (MQ=255)                                                                      acCTTTATGACAGTCAGGGATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTtaat              >  1:159686/1‑90 (MQ=255)                                                                      acCTTTATGACAGTCAGGGATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTtaat              >  2:278927/1‑90 (MQ=255)                                                                       ccTTTATGACAGTCAGGGATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTtaata             >  2:41454/1‑90 (MQ=255)                                                                                 aGTCAGGGATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTg   >  1:11318/1‑90 (MQ=255)                                                                                 aGTCAGGGATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTg   >  2:31384/1‑90 (MQ=255)                                                                                  gTCAGGGATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTgc  >  1:266366/1‑90 (MQ=255)                                                                                        |                                                                                    TCATTACCAATTAAGGCAGTATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGC  >  NZ_CP009273/3347629‑3347799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑