| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,786,467 | T→C | I193V (ATT→GTT) | yibB ← | protein YibB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,786,467 | 0 | T | C | 90.5% | 58.2 / ‑0.2 | 21 | I193V (ATT→GTT) | yibB | protein YibB |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (13/6); total (14/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
TTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGG > NZ_CP009273/3786384‑3786551 | ttCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGaa > 1:354392/1‑90 (MQ=255) aCTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCaaaa < 2:276169/90‑1 (MQ=255) aTTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAATTCAAAAACCt < 2:323290/90‑1 (MQ=255) aaTTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTga < 1:81099/90‑1 (MQ=255) tgtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑t < 2:5346/90‑1 (MQ=255) gtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑tt > 2:346061/1‑90 (MQ=255) tGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTTATAGTGaa < 2:262194/90‑1 (MQ=255) tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTTATAGTGAAAAGATGCATCTTACtt > 1:139696/1‑90 (MQ=255) tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTTATAGTGAAAAGATGCATCTTACtt > 2:272146/1‑90 (MQ=255) tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTTATAGTGAAAAGATGCATCTTACtt > 1:2912/1‑90 (MQ=255) aCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTTATAGTGAAAAGATGCATCTTACTTtca > 2:363522/1‑90 (MQ=255) cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTTATAGTGAAAAGATGCATCTTACTTtcat > 1:62701/1‑90 (MQ=255) tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTTATAGTGAAAAGATGCATCTTACTTtcatca > 2:291686/1‑90 (MQ=255) gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAg < 2:14542/49‑1 (MQ=255) gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAg > 1:14542/1‑49 (MQ=255) gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTTATAGTGAAAAGATGCATCTTACTTtcatca > 2:209464/1‑90 (MQ=255) aTTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaaa > 1:38952/1‑90 (MQ=255) ttGCCAATCATGAAATCAAAAACCTGTTGTTgaga < 1:330081/35‑1 (MQ=255) ttGCCAATCATGAAATCAAAAACCTGTTGTTgaga > 2:330081/1‑35 (MQ=255) ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt > 1:107300/1‑90 (MQ=255) gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATgg > 1:302035/1‑90 (MQ=255) | TTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGG > NZ_CP009273/3786384‑3786551 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |