Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	463,972	A→G	D45G (GAC→GGC)	decR →	DNA‑binding transcriptional regulator DecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	463,972	0	A	G	100.0%	23.8 / NA	8	D45G (GAC→GGC)	decR	DNA‑binding transcriptional regulator DecR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/4);  total (4/4)

GCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAAC  >  NZ_CP009273/463888‑464058                                                                                     |                                                                                       gCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTa                                                                                   >  1:83654/1‑90 (MQ=255)       tctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTa                                                                             >  2:83556/1‑90 (MQ=255)                                 gTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCg                                                   >  2:30702/1‑90 (MQ=255)                                          aCAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATa                                          <  2:147651/90‑1 (MQ=255)                                                   ccTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGc                                 >  2:61518/1‑90 (MQ=255)                                                         tGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAcc                           <  1:178482/90‑1 (MQ=255)                                                                                ggaCGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCa    <  2:195090/90‑1 (MQ=255)                                                                                ggaCGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCa    <  2:309041/90‑1 (MQ=255)                                                                                  acggcgGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAAc  <  1:30702/90‑1 (MQ=255)                                                                                     |                                                                                       GCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAAC  >  NZ_CP009273/463888‑464058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑