Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	475,878	T→C	D96G (GAT→GGT)	tomB ←	Hha toxicity modulator TomB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	475,878	0	T	C	100.0%	44.1 / NA	13	D96G (GAT→GGT)	tomB	Hha toxicity modulator TomB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/9);  total (4/9)

CAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTAT  >  NZ_CP009273/475794‑475956                                                                                     |                                                                               cAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGACCCTGc                                                                           <  2:66275/90‑1 (MQ=255)   aGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGACCCTGCat                                                                         <  2:64406/90‑1 (MQ=255)          cTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGCCCCTGCATATTAATa                                                                  <  1:87301/90‑1 (MQ=255)             gCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGACCCTGCATATTAATACCa                                                               >  1:104724/1‑90 (MQ=255)               aGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGACCCTGCATATTAATACCATa                                                             <  2:45531/90‑1 (MQ=255)                         ttCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGACCCTGCATATTAATACCATAACTACTGaac                                                   <  1:113391/90‑1 (MQ=255)                         ttCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGACCCTGCATATTAATACCATAACTACTGaac                                                   <  1:258943/90‑1 (MQ=255)                            gtcgCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGACCCTGCATATTAATACCATAACTACTGaacaac                                                >  2:210517/1‑90 (MQ=255)                                           caacGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGACCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATcc                                 <  1:285181/90‑1 (MQ=255)                                                        gATTACCTGACTTCCGCCATTTCTGAAGACCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGa                    >  2:355302/1‑90 (MQ=255)                                                                  cTTCCGCCATTTCTGAAGACCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAAt          <  2:104724/90‑1 (MQ=255)                                                                    tCCGCCATTTCTGAAGACCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGa        >  2:8191/1‑90 (MQ=255)                                                                          aTTTCTGAAGACCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTAt  <  1:210517/90‑1 (MQ=255)                                                                                     |                                                                               CAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTAT  >  NZ_CP009273/475794‑475956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑