Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	476,525	A→G	intergenic (‑361/+185)	tomB ← / ← acrB	Hha toxicity modulator TomB/efflux RND transporter permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	476,525	0	A	G	100.0%	20.2 / NA	7	intergenic (‑361/+185)	tomB/acrB	Hha toxicity modulator TomB/efflux RND transporter permease AcrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/4);  total (3/4)

AGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTT  >  NZ_CP009273/476439‑476610                                                                                       |                                                                                      aGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGgtta                                                                                    >  1:11952/1‑90 (MQ=255) aGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGgtta                                                                                    >  1:48657/1‑90 (MQ=255) aGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGgtta                                                                                    >  2:328150/1‑90 (MQ=255)   gATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGgttatt                                                                                  >  1:111000/1‑90 (MQ=255)                 tGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGGTTATTAAATCTATTAgcgc                                                                    <  2:260901/90‑1 (MQ=255)                 tGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGGTTATTAAATCTATTAgcgc                                                                    >  2:3876/1‑90 (MQ=255)                 tGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGGTTATTAAATCTATTAgcgc                                                                    <  2:72461/90‑1 (MQ=255)                              gAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGGTTATTAAATCTATTAGCGCTACTTAATATaaa                                                       >  2:206135/1‑90 (MQ=255)                                     gaatAAAGAATTATCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGGTTATTAAATCTATTAGCGCTACTTAATATAAATtaacta                                                <  2:318494/90‑1 (MQ=255)                                                                   tAGCCTCTAAATGATTATGGGTTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCg                  <  2:248481/90‑1 (MQ=255)                                                                                   aTGGGTTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGttt  >  2:80835/1‑90 (MQ=255)                                                                                       |                                                                                      AGGATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTT  >  NZ_CP009273/476439‑476610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑