| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 661,892 | A→C | G594G (GGT→GGG) | mrdA ← | peptidoglycan DD‑transpeptidase MrdA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 661,892 | 0 | A | C | 85.7% | 39.5 / 1.8 | 15 | coding (1782/1902 nt) | mrdA | peptidoglycan DD‑transpeptidase MrdA |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base C (8/4); minor base . (1/1); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01 | |||||||||||
TGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCACCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTC > NZ_CP009273/661822‑661966 | tgttgtTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGt > 1:244806/1‑75 (MQ=255)tgttgtTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGt < 2:244806/75‑1 (MQ=255) gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt > 1:97917/1‑83 (MQ=255) gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt < 2:97917/83‑1 (MQ=255) gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa > 2:81549/1‑90 (MQ=255) gAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGTGGATTGTTGTATgg > 1:303338/1‑90 (MQ=255) aaCCGCCGGACCCGCACC‑CCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg < 1:283109/53‑1 (MQ=255) aaCCGCCGGACCCGCACC‑CCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg > 2:283109/1‑53 (MQ=255) cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt > 1:26891/1‑53 (MQ=255) cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt < 2:26891/53‑1 (MQ=255) cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAGAGGCGGTCATCAGTTTGTGGTc > 1:10353/1‑90 (MQ=255) cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc > 1:280003/1‑90 (MQ=255) cgccgGACCCGCACCACCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc > 2:316618/1‑90 (MQ=255) ccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTAt > 1:243913/1‑59 (MQ=255) ccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTAt < 2:243913/59‑1 (MQ=255) | TGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCACCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTC > NZ_CP009273/661822‑661966 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |