Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	866,539	C→G	A39A (GCC→GCG)	gsiC →	glutathione ABC transporter permease GsiC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	866,539	0	C	G	100.0%	26.0 / NA	9	A39A (GCC→GCG)	gsiC	glutathione ABC transporter permease GsiC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (4/5);  total (4/5)

TGTTTATCGTCTCGGTGCTGGTGTTTTTATTTGTCCATATGCTGCCCGGCGATCCGGCGCGATTGATTGCCGGGCCCGAAGCTGATGCGCAGGTTATAGAACTGGTGCGTCAGCAGCTGGGGTTGGATCAGCCGCTGTATCACCAGTTCTGGCA  >  NZ_CP009273/866469‑866622                                                                       |                                                                                    tGTTTATCGTCTCGGTGCTGGTGTTTTTATTTGTCCATATGCTGCCCGGCGATCCGGCGCGATTGATTGCGGGGCCCGAAGCTGATgcgc                                                                  <  2:151703/90‑1 (MQ=255)  gTTTATCGTCTCGGTGCTGGTGTTTTTATTTGTCCATATGCTGCCCGGCGATCCGGCGCGATTGATTGCGGGGCCCGAAGCTGATGCGCa                                                                 >  2:298947/1‑90 (MQ=255)   tttATCGTCTCGGTGCTGGTGTTTTTATTTGTCCATATGCTGCCCGGCGATCCGGCGCGATTGATTGCGGGGCCCGAAGCTGATGCGCAg                                                                <  1:77404/90‑1 (MQ=255)          tctcGGTGCTGGTGTTTTTATTTGTCCATATGCTGCCCGGCGATCCGGCGCGATTGATTGCGGGGCCCGAAGCTGATGCGCAGGTTATAg                                                         >  1:109055/1‑90 (MQ=255)          tctcGGTGCTGGTGTTTTTATTTGTCCATATGCTGCCCGGCGATCCGGCGCGATTGATTGCGGGGCCCGAAGCTGATGCGCAGGTTATAg                                                         >  2:326769/1‑90 (MQ=255)                              tttGTCCATATGCTGCCCGGCGATCCGGCGCGATTGATTGCGGGGCCCGAAGCTGATGCGCAGGTTATAGAACTGGTGCGTCAGCAGCTg                                     <  2:144700/90‑1 (MQ=255)                                    cATATGCTGCCCGGCGATCCGGCGCGATTGATTGCGGGGCCCGAAGCTGATGCGCAGGTTATAGAACTGGTGCGTCAGCAGCTGGGGTTg                               <  2:92977/90‑1 (MQ=255)                                           tgcCCGGCGATCCGGCGCGATTGATTGCGGGGCCCGAAGCTGATGCGCAGGTTATAGAACTGGTGCGTCAGCAGCTGGGGTTGGATCAGc                        >  2:35116/1‑90 (MQ=255)                                                                 gattgCGGGGCCCGAAGCTGATGCGCAGGTTATAGAACTGGTGCGTCAGCAGCTGGGGTTGGATCAGCCGCTGTATCACCAGTTCTGGCa  <  2:211432/90‑1 (MQ=255)                                                                       |                                                                                    TGTTTATCGTCTCGGTGCTGGTGTTTTTATTTGTCCATATGCTGCCCGGCGATCCGGCGCGATTGATTGCCGGGCCCGAAGCTGATGCGCAGGTTATAGAACTGGTGCGTCAGCAGCTGGGGTTGGATCAGCCGCTGTATCACCAGTTCTGGCA  >  NZ_CP009273/866469‑866622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑