Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	874,237	C→T	L14F (CTT→TTT)	yliI →	aldose sugar dehydrogenase YliI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	874,237	0	C	T	100.0%	37.8 / NA	11	L14F (CTT→TTT)	yliI	aldose sugar dehydrogenase YliI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/5);  total (6/5)

ATCTCCTCCACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCAT  >  NZ_CP009273/874150‑874305                                                                                        |                                                                     aTCTCCTCCACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGtttt                                                                    <  1:43439/90‑1 (MQ=255)        ccACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCTACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCg                                                             <  1:99563/90‑1 (MQ=255)         cACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGc                                                            <  1:220928/90‑1 (MQ=255)               cTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTg                                                      >  1:359094/1‑90 (MQ=255)                 aCGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTggg                                                    >  1:281496/1‑90 (MQ=255)                            cAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCa                                         >  2:196892/1‑90 (MQ=255)                              gagTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAAc                                       >  1:147191/1‑90 (MQ=255)                                   aaTCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTaa                                  >  2:94441/1‑90 (MQ=255)                                    aTCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTaaa                                 >  1:166164/1‑90 (MQ=255)                                             cGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGt                        <  2:144567/90‑1 (MQ=255)                                                                  tttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCa   <  2:235229/90‑1 (MQ=255)                                                                   ttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCAt  <  2:281496/90‑1 (MQ=255)                                                                                        |                                                                     ATCTCCTCCACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCAT  >  NZ_CP009273/874150‑874305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑