Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	991,969	T→G	L144F (TTA→TTC)	ssuA ←	aliphatic sulfonate ABC transporter substrate‑binding protein SsuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	991,969	0	T	G	100.0%	35.1 / NA	11	L144F (TTA→TTC)	ssuA	aliphatic sulfonate ABC transporter substrate‑binding protein SsuA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/5);  total (6/5)

GGGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTC  >  NZ_CP009273/991896‑992052                                                                          |                                                                                    gggCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAATGCACGCAGGAAAAGGGTGTGTGAAc                                                                     >  1:167643/1‑90 (MQ=255)  ggCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACt                                                                    <  1:176394/90‑1 (MQ=255)            ggCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTc                                                          >  1:242748/1‑90 (MQ=255)                             ttGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTtgtg                                         >  2:340076/1‑90 (MQ=255)                              tGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGa                                        <  1:340076/90‑1 (MQ=255)                                gATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGAcc                                      <  2:115744/90‑1 (MQ=255)                                              tAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTa                        >  1:350852/1‑90 (MQ=255)                                                              cAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTCTGATCGGGCt        <  1:277517/90‑1 (MQ=255)                                                                gTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCa                                                 <  1:45726/47‑1 (MQ=255)                                                                gTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCa                                                 >  2:45726/1‑47 (MQ=255)                                                                    aCGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTc  >  2:252171/1‑90 (MQ=255)                                                                          |                                                                                    GGGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTC  >  NZ_CP009273/991896‑992052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑