Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,110,952	T→C	intergenic (‑6/+166)	mdtG ← / ← lpxL	multidrug efflux MFS transporter MdtG/kdo(2)‑lipid IV(A) lauroyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,110,952	0	T	C	100.0%	23.8 / NA	8	intergenic (‑6/+166)	mdtG/lpxL	multidrug efflux MFS transporter MdtG/kdo(2)‑lipid IV(A) lauroyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/4);  total (4/4)

CGATCAGGTTTCGTTTCCAGTTTATAGGGGTGTCATTTTCACAGGGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGC  >  NZ_CP009273/1110895‑1111033                                                          |                                                                                  cgaTCAGGTTTCGTTTCCAGTTTATAGGGGTGTCATTTTCACAGGGTGACATAGCAACCCGCTGTTGGTGCGCCAGGCGCGGTGAACATa                                                   >  1:229911/1‑90 (MQ=255)                                            gggTGACATAGCAACCCGCTGTTGGTGCGCCAGgcgc                                                             >  1:151546/1‑37 (MQ=38)                                            gggTGACATAGCAACCCGCTGTTGGTGCGCCAGgcgc                                                             <  2:151546/37‑1 (MQ=38)                                            gggTGACATAGCAACCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAgg        >  2:104964/1‑90 (MQ=255)                                              gTGACATAGCAACCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCg                 >  1:338830/1‑79 (MQ=255)                                              gTGACATAGCAACCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCg                 <  2:338830/79‑1 (MQ=255)                                                  cATAGCAACCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGgcgc  <  1:232062/90‑1 (MQ=255)                                                  cATAGCAACCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGgcgc  <  1:54274/90‑1 (MQ=255)                                                          |                                                                                  CGATCAGGTTTCGTTTCCAGTTTATAGGGGTGTCATTTTCACAGGGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGC  >  NZ_CP009273/1110895‑1111033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑