Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	98,192	T→C	I314T (ATT→ACT)	murC →	UDP‑N‑acetylmuramate‑‑L‑alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	98,192	0	T	C	100.0%	24.1 / NA	8	I314T (ATT→ACT)	murC	UDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/1);  total (7/1)

GCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAG  >  NZ_CP009273/98115‑98266                                                                              |                                                                           gcCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCt                                                                >  2:356478/1‑90 (MQ=255)     ggTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGaa                                                            >  2:254926/1‑90 (MQ=255)                     tGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTg                                            <  1:237732/90‑1 (MQ=255)                                          cggttgcTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCg                       >  1:185045/1‑90 (MQ=255)                                              tgcTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGa                   >  1:221615/1‑90 (MQ=255)                                                          gggCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGa       >  2:339082/1‑90 (MQ=255)                                                               ttGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAg  >  1:325468/1‑90 (MQ=255)                                                               ttGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAg  >  2:223696/1‑90 (MQ=255)                                                                              |                                                                           GCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAG  >  NZ_CP009273/98115‑98266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑