Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,320,565	G→A	Q521Q (CAG→CAA)	yciQ →	DUF2207 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,320,565	0	G	A	100.0%	22.3 / NA	7	Q521Q (CAG→CAA)	yciQ	DUF2207 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/6);  total (1/6)

AGCGGGTTATTTTACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACAC  >  NZ_CP009273/1320478‑1320626                                                                                        |                                                              aGCGGGTTATTTTACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGacaaca                                                             >  2:155760/1‑90 (MQ=255)  gCGGGTTATTTTACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGAcaacaa                                                            >  1:159207/1‑90 (MQ=255)            ttACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGccc                                                  <  1:6392/90‑1 (MQ=255)                                                  gggTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAagag            <  1:290915/90‑1 (MQ=255)                                                    gTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGgc          <  1:144838/90‑1 (MQ=255)                                                    gTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGgc          <  2:259334/90‑1 (MQ=255)                                                            ttCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAacac  <  2:159207/90‑1 (MQ=255)                                                              cTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGc                                        >  1:120824/1‑50 (MQ=255)                                                              cTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGc                                        <  2:120824/50‑1 (MQ=255)                                                                                        |                                                              AGCGGGTTATTTTACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACAC  >  NZ_CP009273/1320478‑1320626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑