Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	108,362	A→G	F242S (TTC→TCC)	zapD ←	cell division protein ZapD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	108,362	0	A	G	100.0%	35.7 / NA	11	F242S (TTC→TCC)	zapD	cell division protein ZapD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/3);  total (8/3)

CACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTG  >  NZ_CP009273/108283‑108447                                                                                |                                                                                      caccacCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACg                                                                             >  2:237826/1‑90 (MQ=255)     accGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTcc                                                                         <  2:116290/90‑1 (MQ=255)                      tGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTc                                                        <  2:301108/90‑1 (MQ=255)                                 ccGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCg                                             >  1:178461/1‑90 (MQ=255)                                 ccGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCg                                             >  2:184451/1‑90 (MQ=255)                                             cTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGaa                                 >  1:97000/1‑90 (MQ=255)                                                             ttAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCtt                 >  1:246922/1‑90 (MQ=255)                                                             ttAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCtt                 >  2:30121/1‑90 (MQ=255)                                                                   aCAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTcc           >  2:157616/1‑90 (MQ=255)                                                                           gTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAttt   >  1:192919/1‑90 (MQ=255)                                                                            ttCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTg  <  2:27343/90‑1 (MQ=255)                                                                                |                                                                                      CACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTG  >  NZ_CP009273/108283‑108447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑