Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,402,000	A→G	I18T (ATC→ACC)	dgcM ←	diguanylate cyclase DgcM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,402,000	0	A	G	100.0%	32.0 / NA	10	I18T (ATC→ACC)	dgcM	diguanylate cyclase DgcM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/6);  total (4/6)

CGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTATTTTTATCAATTTTTGTTGC  >  NZ_CP009273/1401916‑1402085                                                                                     |                                                                                      cGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAg                                                                                  <  2:317067/90‑1 (MQ=255)       tGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGa                                                                            >  1:264737/1‑90 (MQ=255)        gACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGAc                                                                           <  1:186020/90‑1 (MQ=255)        gACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGAc                                                                           >  2:242756/1‑90 (MQ=255)        gACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGAc                                                                           >  2:95786/1‑90 (MQ=255)                gTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGt                                                                   <  1:334655/90‑1 (MQ=255)                        ttGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAg                                                           <  2:25121/90‑1 (MQ=255)                                          cgcgCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGt                                         <  2:342757/90‑1 (MQ=255)                                           gcgcTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTa                                        >  2:179002/1‑90 (MQ=255)                                                                                 aaCGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTATTTTTATCAATTTTTGTTGc  <  1:350066/90‑1 (MQ=255)                                                                                     |                                                                                      CGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTATTTTTATCAATTTTTGTTGC  >  NZ_CP009273/1401916‑1402085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑