Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,454,774	A→G	D16G (GAT→GGT)	paaI →	hydroxyphenylacetyl‑CoA thioesterase PaaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,454,774	0	A	G	100.0%	42.0 / NA	13	D16G (GAT→GGT)	paaI	hydroxyphenylacetyl‑CoA thioesterase PaaI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/5);  total (8/5)

CTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAA  >  NZ_CP009273/1454697‑1454857                                                                              |                                                                                    ctgcGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCaa                                                                         <  1:304784/90‑1 (MQ=255)   gcgcCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTAGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAg                                                                       >  2:216862/1‑90 (MQ=255)        aaCGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCtt                                                                  >  2:173608/1‑90 (MQ=255)                          gCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCa                                                >  2:59955/1‑90 (MQ=255)                                      cATAAGGCCTGGCAAAATGCCCATGCAATGTTTGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc                                    <  1:188451/90‑1 (MQ=255)                                      cATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc                                    <  1:154824/90‑1 (MQ=255)                                       aTAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCt                                   >  1:274122/1‑90 (MQ=255)                                       aTAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCt                                   >  2:180381/1‑90 (MQ=255)                                              cTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGt                            >  1:236246/1‑90 (MQ=255)                                                    aaaTGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGAc                      >  2:21649/1‑90 (MQ=255)                                                    aaaTGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTCGCTGTAGTGAc                      <  2:351277/90‑1 (MQ=255)                                                                aaTGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCAc          <  2:115638/90‑1 (MQ=255)                                                                        agaACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACaaa  >  2:174788/1‑90 (MQ=255)                                                                              |                                                                                    CTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAA  >  NZ_CP009273/1454697‑1454857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑