Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,503,965	T→C	D67D (GAT→GAC)	hicB →	type II toxin‑antitoxin system antitoxin HicB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,503,965	0	T	C	100.0%	12.6 / NA	5	D67D (GAT→GAC)	hicB	type II toxin‑antitoxin system antitoxin HicB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/4);  total (1/4)

CTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGA  >  NZ_CP009273/1503889‑1503987                                                                             |                       cTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAg           <  1:178506/90‑1 (MQ=255) cTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAg           <  1:179790/90‑1 (MQ=255) cTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAg           <  2:280268/90‑1 (MQ=255)  tttACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGt          <  2:71671/90‑1 (MQ=255)          ccGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGa  >  2:310668/1‑90 (MQ=255)                                                                             |                       CTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGA  >  NZ_CP009273/1503889‑1503987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑