Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,505,430	A→G	K391E (AAA→GAA)	ydcR →	PLP‑dependent aminotransferase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,505,430	0	A	G	100.0%	31.0 / NA	10	K391E (AAA→GAA)	ydcR	PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/7);  total (3/7)

CCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGC  >  NZ_CP009273/1505345‑1505495                                                                                      |                                                                  ccATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAAt                                                               <  1:41810/90‑1 (MQ=255)  cATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTTTCTGCCTGCGGAAGTGGAAAtt                                                              >  2:119711/1‑90 (MQ=255)  cATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAAtt                                                              >  2:119710/1‑90 (MQ=255)      ttCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGCCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATtcatc                                                          <  1:329780/90‑1 (MQ=255)           cgCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAAt                                                     <  1:242058/90‑1 (MQ=255)              cTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGAc                                                  >  1:188960/1‑90 (MQ=255)                             gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTTTCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTtctc                                   <  1:119711/90‑1 (MQ=255)                             gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTtctc                                   <  1:119710/90‑1 (MQ=255)                                       aacaacGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGc                         <  1:141802/90‑1 (MQ=255)                                                              ctgcTTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGc  <  1:138706/90‑1 (MQ=255)                                                                                      |                                                                  CCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGC  >  NZ_CP009273/1505345‑1505495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑