Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,558,439	A→G	Y518H (TAT→CAT)	dosP ←	oxygen‑sensing cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,558,439	0	A	G	100.0%	32.5 / NA	10	Y518H (TAT→CAT)	dosP	oxygen‑sensing cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/6);  total (4/6)

TGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGTAAGCG  >  NZ_CP009273/1558353‑1558522                                                                                       |                                                                                    tGCGCCTAAAACAAAACGTTTTTTTCCCTTTCCGTTCATCGCGGGGTGGAAGAATTGCCAGCCGTTACCGCCATTCTTGCGAATATGATc                                                                                  <  2:85956/90‑1 (MQ=255) tGCGCCAAAACCCAACCGCTTTTTTCCCTTTTCTTTCATCGCGGGGTTAAAGACCTGCCAGCCGTTACCGCCATTCTTGCGAATATGATc                                                                                  <  2:341095/90‑1 (MQ=255)  gcgcCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATcc                                                                                 >  2:139902/1‑90 (MQ=255)              aaaCGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGa                                                                     >  1:97126/1‑90 (MQ=255)                             aTTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAg                                                      >  1:8764/1‑90 (MQ=255)                                               cTGAAGACCTCCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTAccc                                    <  1:234397/90‑1 (MQ=255)                                               cTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTAccc                                    <  2:308447/90‑1 (MQ=255)                                                         gCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGc                          <  2:97126/90‑1 (MQ=255)                                                                              tGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGTaa     <  1:128857/90‑1 (MQ=255)                                                                                 gAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGTAAGCg  >  2:350325/1‑90 (MQ=255)                                                                                       |                                                                                    TGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGTAAGCG  >  NZ_CP009273/1558353‑1558522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑