Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,595,655	A→G	intergenic (‑157/‑92)	lsrR ← / → lsrA	transcriptional regulator LsrR/autoinducer 2 ABC transporter ATP‑binding protein LsrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,595,655	0	A	G	100.0%	28.1 / NA	9	intergenic (‑157/‑92)	lsrR/lsrA	transcriptional regulator LsrR/autoinducer 2 ABC transporter ATP‑binding protein LsrA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/4);  total (5/4)

AACCACAGTTTGACTCTACGAGCATGAACAAACGCAACCGTGAAAATCAAAATAGCATAAATTGTGATCTATTCGTCGGAAATATGTGCAATGTCCACCTAAGGTTATGAACAAATTAAAAGCAGAAATACATTTGTTCAAAACTCACCTGCAAAACTGAAC  >  NZ_CP009273/1595576‑1595737                                                                                |                                                                                   aaCCACAGTTTGACTCTACGAGCATGAACAAACGCAACCGTGAAAATCAAAATAGCATAAATTGTGATCTATTCGTCGGGAATATGTGCa                                                                          >  1:290039/1‑90 (MQ=255)  aCCACAGTTTGACTCTACGAGCATGAACAAACGCAACCGTGAAAATCAAAATAGCATAAATTGTGATCTATTCGTCGGGAATATGTGCaa                                                                         <  1:77354/90‑1 (MQ=255)                ctACGAGCATGAACAAACGCAACCGTGAAAATCAAAATAGCATAAATTGTGATCTATTCGTCGGGAATATGTGCAATGTCCACCTaa                                                              >  1:116457/1‑87 (MQ=255)                ctACGAGCATGAACAAACGCAACCGTGAAAATCAAAATAGCATAAATTGTGATCTATTCGTCGGGAATATGTGCAATGTCCACCTaa                                                              <  2:116457/87‑1 (MQ=255)                        aTGAACAAACGCAACCGTGAAAATCAAAATAGCATAAATTGTGATCTATTCGTCGGGAATATGTGCAATGTCCACCTAAGGTTATGAACa                                                   >  1:68334/1‑90 (MQ=255)                               aaCGCAACCGTGAAAATCAAAATAGCATAAATTGTGATCTATTCGTCGGGAATATGTGCAATGTCCACCTAAGGTTATGAACAAATTaaa                                            >  1:165599/1‑90 (MQ=255)                                                       gCATAAATTGTGATCTATTCGTCGGGAATATGTGCAATGTc                                                                     >  1:224649/1‑41 (MQ=255)                                                       gCATAAATTGTGATCTATTCGTCGGGAATATGTGCAATGTc                                                                     <  2:224649/41‑1 (MQ=255)                                                                         tcgtcgGGAATATGTGCAATGTCCACCTAAGGTTATGAACAAATTAAAAGCAGAAATACATTTGTTCAAAACTCACCTGCAAAACTGAAc  <  1:309739/90‑1 (MQ=255)                                                                                |                                                                                   AACCACAGTTTGACTCTACGAGCATGAACAAACGCAACCGTGAAAATCAAAATAGCATAAATTGTGATCTATTCGTCGGAAATATGTGCAATGTCCACCTAAGGTTATGAACAAATTAAAAGCAGAAATACATTTGTTCAAAACTCACCTGCAAAACTGAAC  >  NZ_CP009273/1595576‑1595737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑