Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,240,670	Δ1 bp	coding (55/441 nt)	BW25113_RS06225 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,240,667	0	G	.	100.0%	28.2 / NA	8	coding (52/441 nt)	BW25113_RS06225	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (6/2);  total (6/2)

GAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAG  >  NZ_CP009273/1240580‑1240734                                                                                        |                                                                    gAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATggg                                                                   <  2:194184/90‑1 (MQ=255)           ttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGa                                                        >  2:381820/1‑90 (MQ=255)                      ttCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTgg                                             >  2:80330/1‑90 (MQ=255)                                              aaaaTAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAAt                     <  1:102086/90‑1 (MQ=255)                                                       atTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTTTTGAACATTTAGAATTGATCAATc            >  2:359201/1‑90 (MQ=255)                                                                 gTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTTGCTGGGCTTATTTAAAATTTAGAATTGATCAATACTTCTGGCAg  >  1:200616/1‑90 (MQ=255)                                                                 gTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTTGCAg  >  1:176058/1‑90 (MQ=255)                                                                      cAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGa                                >  1:174631/1‑55 (MQ=255)                                                                      cAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGa                                <  2:174631/55‑1 (MQ=255)                                                                                        |                                                                    GAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAG  >  NZ_CP009273/1240580‑1240734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑