Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,276,251	Δ1 bp	coding (932/3744 nt)	narG →	nitrate reductase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,276,249	0	G	.	100.0%	27.6 / NA	8	coding (930/3744 nt)	narG	nitrate reductase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (5/3);  total (5/3)

CACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACC  >  NZ_CP009273/1276162‑1276324                                                                                        |                                                                            cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggc                                                                           <  1:384709/90‑2 (MQ=255)                             cTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCg                                              <  1:12552/90‑1 (MQ=255)                               gtgCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGAc                                            >  1:135479/1‑90 (MQ=255)                                      cTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCaa                                     >  1:136736/1‑90 (MQ=255)                                                   cGAAACAGGGCCCCGCTGCGGCAATGGCGCTGGCGAT‑GGCCCCGTAATGCTGCGTGAATTCCACCTCGACACCCCAAGCCAGTATTTCAc                        <  2:246888/90‑1 (MQ=255)                                                    gAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggcc                                                                          >  1:96163/1‑38 (MQ=255)                                                    gAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggcc                                                                          <  2:96163/40‑3 (MQ=255)                                                          gggCACCGATGCCGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAt                 >  2:49575/1‑90 (MQ=255)                                                              aCCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGc             <  1:405823/90‑1 (MQ=255)                                                               ccGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCg            >  2:123525/1‑90 (MQ=255)                                                                         aaTGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACAcc  >  2:255274/1‑90 (MQ=255)                                                                                        |                                                                            CACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACC  >  NZ_CP009273/1276162‑1276324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑