Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,632,437	(T)7→6	intergenic (‑71/+8)	BW25113_RS08135 ← / ← BW25113_RS25620	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,632,431	0	T	.	100.0%	32.5 / NA	9	intergenic (‑65/+14)	BW25113_RS08135/BW25113_RS25620	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (4/5);  total (4/5)

TTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCG  >  NZ_CP009273/1632350‑1632497                                                                                  |                                                                   tttGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTCCTGGTTCAGAGTTAAAAAAACTATGAttttttcat                                                            <  1:90929/90‑4 (MQ=255)            aCGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAGACTATGA‑TTTTTTCATGATGTTACCgt                                                <  1:77121/90‑1 (MQ=255)            aCGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                <  1:66198/90‑1 (MQ=255)            aCGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                <  2:25072/90‑1 (MQ=255)              gCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgtag                                              <  1:318911/90‑1 (MQ=255)                        gtTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTa                                    >  1:56315/1‑90 (MQ=255)                             cTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCAt                               >  1:128152/1‑90 (MQ=255)                                                       gctgGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                <  1:402612/47‑1 (MQ=39)                                                       gctgGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                >  2:402612/1‑47 (MQ=39)                                                          ggTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCg  >  1:223487/1‑90 (MQ=255)                                                                                  |                                                                   TTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCG  >  NZ_CP009273/1632350‑1632497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑