| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,751,028 | 0 | C | T | 53.8% | 1.0 / 11.9 | 13 | A54T (GCA→ACA) | yiaY | L‑threonine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (4/2); new base T (0/7); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGC > NZ_CP009273/3750948‑3751109 | aCCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGcac < 2:258043/90‑1 (MQ=255)aCCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGcac < 2:353983/90‑1 (MQ=255) ggggTTAGGTTGGGTGCCATCAAAAATAACGCTAAAAATATTGCGTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGt < 2:283068/90‑1 (MQ=255) ttAGGTGGGGTGCCATCAAAAATAACGCAAAAAATATTGCTTTCTTCCAGTTTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAAc < 1:101719/90‑1 (MQ=255) gggTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGt > 1:204424/1‑90 (MQ=255) gggTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGt > 2:190572/1‑90 (MQ=255) ggTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTc > 1:189584/1‑90 (MQ=255) ccATCATAAAAAACGCTAAAAATTTTGCTTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTg < 2:247474/90‑1 (MQ=255) ccATCAAAACAAACGCAAAAATTTTTGCTTTCTTCCATTGTTTTTTGCACCCCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTg < 1:317639/90‑1 (MQ=255) aaataaCACAAAAAATTTTGCTTTCTTCCAGTTTTTTTTCCACATCCCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTa < 1:309957/90‑1 (MQ=255) gCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTAcc > 2:7556/1‑89 (MQ=255) ttcCATTGTTTTTTCCACACCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGc < 1:190572/90‑1 (MQ=255) ttcCAGTGTTTTTTGCACACCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGc < 2:321918/90‑1 (MQ=255) | ACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGC > NZ_CP009273/3750948‑3751109 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |