| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,224,160 | 0 | G | T | 53.3% | 6.4 / 12.9 | 15 | D159Y (GAC→TAC) | pgi | glucose‑6‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base G (2/5); new base T (8/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTC > NZ_CP009273/4224079‑4224247 | gAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGc < 1:110135/90‑1 (MQ=255) gATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTTTTACCTCCGCCCCTa > 2:392962/1‑90 (MQ=255) gATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGGTCTGACCCCGGccccca > 2:80216/1‑87 (MQ=255) tCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGGTCTGGCCCCCGCCCCAACATGGTg > 1:226501/1‑90 (MQ=255) gAGTGGAAAGGTTATACCGGCAAAGCGATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGaa < 1:80516/90‑1 (MQ=255) gTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGc < 1:184173/90‑1 (MQ=255) gTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTTTTGCCTCCGCCCCCACAAGGTTACCGCAGCTCTGCggc > 1:116993/1‑88 (MQ=255) aCTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAAc < 1:300312/90‑1 (MQ=255) aCGTAGTGAACATCGGGATCGGCGGTTTTTACCTCCGCCCCCACAAGGTGGCCGGAGATCCGCGTCCGGGCAAAAAACACCCGAAAATGc > 1:128597/1‑90 (MQ=255) aCGTAGTGAACATCGGGATCGGCGGTTTTTACCTCCGCCCATACATGGGGACCGAAGCTCTGCGTCCGTACAAAAAACACCCGAACAAGc > 2:162843/1‑90 (MQ=255) aCGTAGTGAACATCGGGATCGGCGGTTCTTACCTCCGCCCCCACAAGGGGTCCCAAACCCTGCCCCCCCACAAAAAACACCCCAACAAGc > 1:176027/1‑90 (MQ=255) aGTGAACATCGGGATCGGCGGTTTTTACCCCCGCCCCCACATGGGGGCCCAAACTCTGCGTCCGTACAAAAAACACCCCAACAAGGACtt > 1:43716/1‑90 (MQ=255) aTCGGGATCGGCGGTTTTTACCCCCGCCCCTACAAGGGGGCCGAAACTCTGCGTCCCTACAAAAAACACCCGAACAAGCACTtttttttt > 2:237070/1‑88 (MQ=255) gggATCGGCGGTTTTTACCCCGGCCCCTAAATGGGGGCCGAAGCTCTGCGTCCGTGCAAAAAACACCCGAACATGCACTTTTTTTTTAAc > 1:1061/1‑90 (MQ=255) cTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTc < 2:43716/90‑1 (MQ=255) | GAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTC > NZ_CP009273/4224079‑4224247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |