| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,224,171 | 0 | A | C | 57.1% | ‑0.6 / 12.1 | 14 | P162P (CCA→CCC) | pgi | glucose‑6‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base C (8/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGC > NZ_CP009273/4224084‑4224254 | gATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTTTTACCTCCGCCCCTa > 2:392962/1‑90 (MQ=255) tCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGGTCTGGCCCCCGCCCCAACATGGTg > 1:226501/1‑90 (MQ=255) gAGTGGAAAGGTTATACCGGCAAAGCGATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGaa < 1:80516/90‑1 (MQ=255) gTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGc < 1:184173/90‑1 (MQ=255) gTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTTTTGCCTCCGCCCCCACAAGGTTACCGCAGCTCTGCggc > 1:116993/1‑88 (MQ=255) aCTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAAc < 1:300312/90‑1 (MQ=255) aCGTAGTGAACATCGGGATCGGCGGTTTTTACCTCCGCCCCCACAAGGTGGCCGGAGATCCGCGTCCGGGCAAAAAACACCCGAAAATGc > 1:128597/1‑90 (MQ=255) aCGTAGTGAACATCGGGATCGGCGGTTTTTACCTCCGCCCATACATGGGGACCGAAGCTCTGCGTCCGTACAAAAAACACCCGAACAAGc > 2:162843/1‑90 (MQ=255) aCGTAGTGAACATCGGGATCGGCGGTTCTTACCTCCGCCCCCACAAGGGGTCCCAAACCCTGCCCCCCCACAAAAAACACCCCAACAAGc > 1:176027/1‑90 (MQ=255) aGTGAACATCGGGATCGGCGGTTTTTACCCCCGCCCCCACATGGGGGCCCAAACTCTGCGTCCGTACAAAAAACACCCCAACAAGGACtt > 1:43716/1‑90 (MQ=255) aTCGGGATCGGCGGTTTTTACCCCCGCCCCTACAAGGGGGCCGAAACTCTGCGTCCCTACAAAAAACACCCGAACAAGCACTtttttttt > 2:237070/1‑88 (MQ=255) gggATCGGCGGTTTTTACCCCGGCCCCTAAATGGGGGCCGAAGCTCTGCGTCCGTGCAAAAAACACCCGAACATGCACTTTTTTTTTAAc > 1:1061/1‑90 (MQ=255) cTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTc < 2:43716/90‑1 (MQ=255) cGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATcgc > 2:217757/1‑90 (MQ=255) | GATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGC > NZ_CP009273/4224084‑4224254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |