| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,464,532 | 0 | T | G | 60.0% | 8.5 / 10.5 | 15 | *198G (TGA→GGA) | bdcR | TetR/AcrR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (4/2); new base G (9/0); total (13/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTG > NZ_CP009273/4464443‑4464620 | cTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGt < 2:223334/90‑1 (MQ=255) ctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGGGGGATGCGGGCCTTTACCCC‑AAAGGc > 2:99666/1‑90 (MQ=255) ataGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGGGGGATGCGGGGCTTGACCCG‑AAAGGCgg > 1:162665/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCG‑AAAGGCGGGAAAacgc > 2:367127/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGGGGGATGCGGGCCTTGACCCG‑AAAGGCGGGGAAacgc > 1:52680/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTTTTGAGGAGGGATGCGGGCCTTGACCCG‑AAAGGCGGGGAAgcgc > 2:328879/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCG‑AAAGGCGGGAAAgcgc > 2:45948/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCG‑AAAGGCGGGAAAgcgc > 2:90161/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTCTTGAGGAGGGAAGCGGGCCTTGACCCGAAAAGGGGGGaaaagc > 1:110876/1‑87 (MQ=255) gtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTTTTGAGGAGTGATGCGGGCCTTTACCCG‑AAAGGCGGGGAAACGCCTGcc > 2:327265/1‑90 (MQ=255) cAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTAGGGGGGATGCGGGCCTTGACCCG‑AAAGGCGGGGAAGCGCCCGCCGAt > 1:351127/1‑90 (MQ=255) aaCAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATa < 2:168419/90‑1 (MQ=255) ggagTGATGCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATa < 1:252819/58‑1 (MQ=255) ggagTGATGCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATa > 2:252819/1‑58 (MQ=255) agTGATGCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAAtt > 1:369903/1‑90 (MQ=255) gTGATGCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGGTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTg > 1:214295/1‑90 (MQ=255) | CTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTG > NZ_CP009273/4464443‑4464620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |