Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,512,8230AC57.1% 10.3 / 14.1 14G363G (GGT→GGG) yjhGxylonate dehydratase YjhG
Reads supporting (aligned to +/- strand):  ref base A (5/1);  new base C (0/8);  total (5/9)
Fisher's exact test for biased strand distribution p-value = 3.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03
Rejected as consensus: Frequency below/above cutoff threshold.

CCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCG  >  NZ_CP009273/4512759‑4512897
                                                                |                                                                          
ccGTCATACCTTCTCCCCCCAACACCCCGACGCTGCGCAGATCCAACATGCCTCCCGGCCCCCCACCTGCCATAAAGGCATTGACCGTTg                                                   <  1:191700/90‑1 (MQ=255)
ccGTCATACCGTCTTAATGCAACAACCCGAGGCTGCGCAGATGCAACATGCCTTCCGCCCCACCCCCTGCCATAAAGGCATTGACCGTTg                                                   <  1:199398/90‑1 (MQ=255)
ccGTCAAACCGTCTTCATGCAACAACCCGAGGCTGCGCAGATGAAACATGACTTCCGGCACACCCCCTGCCATAAAGGCATTGACCGTTg                                                   <  1:376013/90‑1 (MQ=255)
            cTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCg                                       >  2:341893/1‑90 (MQ=255)
             ttCATGCAACAACCCGAGGCTGCGCAGATGCAACATGCCTCCCGGCACCCCCCCTGCCATAAAGGCATTGACCGTTGGATGATAAACCgg                                      <  1:180307/90‑1 (MQ=255)
             ttCATGCAACAACCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCCCCTGCCATAAAGGCATTGACCGTTGGATGATAAACCgg                                      <  1:4750/90‑1 (MQ=255)
               cATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTCCCGGCACCCCCCCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGc                                    <  1:341893/90‑1 (MQ=255)
                        aTCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACCCCCCCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCa                           <  2:338982/90‑1 (MQ=255)
                                  gcgcAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCAc                 >  2:146632/1‑90 (MQ=255)
                                  gcgcAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCAc                 >  2:218246/1‑90 (MQ=255)
                                  gcgcAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCAc                 >  2:38488/1‑90 (MQ=255)
                                             aCATGACTTCCGGCACACCCCCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCa      <  2:92583/90‑1 (MQ=255)
                                              cATGACTTCCGGCACCCCCCCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCAc     <  2:403714/90‑1 (MQ=255)
                                                 gACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCAcgcg  >  2:276966/1‑90 (MQ=255)
                                                                |                                                                          
CCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCG  >  NZ_CP009273/4512759‑4512897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑