| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,544,923 | 0 | C | A | 54.5% | ‑1.0 / 10.1 | 11 | N177K (AAC→AAA) | uxuR | Uxu operon transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (1/4); new base A (6/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGC > NZ_CP009273/4544834‑4545012 | gACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAc < 1:60679/90‑1 (MQ=255) ccATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAAtgtg < 1:116523/90‑1 (MQ=255) ccATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAAtgtg < 1:364517/90‑1 (MQ=255) gCTATTGCCGAAGCAATGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAAAGTGGGTCCAg > 1:350368/1‑90 (MQ=255) cTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGt < 2:217046/90‑1 (MQ=255) gCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGcc > 2:71017/1‑90 (MQ=255) gCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGTGGATCCAGTTGCACAGCCATCTGGGTGACAGcc > 2:275371/1‑90 (MQ=255) ggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGTGGGTCCAGTTGCACAACCATCTGGGTGACAGCCTGTATTGcca > 2:277119/1‑88 (MQ=255) gTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATGTGGGTCCAGTTGCACAGCCATCTGGATGAAAGCCTGTATCGCAAAAAGGGGGTgg > 2:3150/1‑90 (MQ=255) gTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGa < 1:318431/90‑1 (MQ=255) gTGGCGGGAAAACAAACCAATGTGGGTCCAGGTGCACAGCCATCTGGATGGCAGCCCGTATCGCAAAAAGGGGGTGGGGGGTCACAAACa > 2:318431/1‑90 (MQ=255) tGGCGGGGAAAAAAAACAAAGTGGGGCCAGTTTCACAACCATCTGGATGACAGCCCGTATCGCAAAAAGGGGGTGGGGGGTCACcaaaaa > 1:37719/1‑87 (MQ=255) cAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTcgccgc > 2:190150/1‑90 (MQ=255) | GACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGC > NZ_CP009273/4544834‑4545012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |