| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,558,321 | 0 | T | G | 52.4% | 17.9 / 28.5 | 21 | T6P (ACC→CCC) | mdtM | multidrug efflux MFS transporter MdtM |
| Reads supporting (aligned to +/- strand): ref base T (1/9); new base G (11/0); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCG > NZ_CP009273/4558234‑4558394 | cAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaa > 1:126409/1‑90 (MQ=255) ggCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaa > 2:402725/1‑90 (MQ=255) ggCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaa > 2:380553/1‑90 (MQ=255) ggATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaaaaaa > 2:326066/1‑89 (MQ=255) gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaaaaaaa > 2:51650/1‑88 (MQ=255) aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCAt < 1:51650/90‑1 (MQ=255) ccGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATaa < 1:318498/90‑1 (MQ=255) gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAAAGGCATaaaa > 1:80473/1‑88 (MQ=255) aCGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCt < 1:91266/90‑1 (MQ=255) aCGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCt < 1:128662/90‑1 (MQ=255) gcaAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGAGGCCAAAAAAAAAACACCtttctc > 1:195480/1‑86 (MQ=255) aTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTGAAAAAAATTGGCCTAAAAAACAAACCCTTGTCCGGGGAA‑CCCCCACgg > 2:217027/1‑90 (MQ=255) aTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAATTTGCCCAACAAAAAAACCCTTTTCCCGCGGA‑CCCCaaacg > 2:264593/1‑87 (MQ=255) aTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGGATAAAAAAAAACTCCTTGTCCGGAGAA‑CCCCCACgg > 2:317898/1‑90 (MQ=255) aCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAAAGGCATAAAAAAAAACACCTTGTTCGGAGAAGCCCCACCGGGGa > 1:345874/1‑90 (MQ=255) aTCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGa < 1:380553/90‑1 (MQ=255) aTCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGa < 1:402725/90‑1 (MQ=255) aTCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGa < 1:125900/90‑1 (MQ=255) cGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGaaa < 1:264729/90‑1 (MQ=255) cGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGAAATTCAATACCGTc < 2:345874/90‑1 (MQ=255) gTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAAAAAAAAACCCCTTGTCCGGGGAA‑ACCCCACGGGGGAAAAGAAAATAAAAAACGTCg > 1:10802/1‑90 (MQ=255) | CAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCG > NZ_CP009273/4558234‑4558394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |