Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,854,126	T→C	M121V (ATG→GTG)	ydjK ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,854,126	0	T	C	100.0%	28.6 / NA	10	M121V (ATG→GTG)	ydjK	MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/9);  total (1/9)

CACGTTCCACGATTTCTACCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCATCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAAT  >  NZ_CP009273/1854044‑1854198                                                                                   |                                                                         cACGTTCCACGATTTCTACCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACaaaa                                                                   <  1:259102/90‑1 (MQ=255)       ccACGATTTCTACCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACggcag                                                             <  1:346453/90‑1 (MQ=255)       ccACGATTTCTACCGGCCATATACCCGGTGAAACCAGCAAACCGCCTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACggcag                                                             <  1:206197/90‑1 (MQ=255)                 tACCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTTCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGa                                                   <  2:198978/90‑1 (MQ=255)                            tatTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTc                                        <  1:247695/90‑1 (MQ=255)                            tatTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTc                                        <  1:54613/90‑1 (MQ=255)                                       aCCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGc                             <  1:309617/90‑1 (MQ=255)                                             aaaCAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCAACCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGCACCGAc                       <  2:182345/90‑1 (MQ=255)                                                                 cTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGCACCGACAACCATCGAGGCAATATGaa   >  2:122233/1‑90 (MQ=255)                                                                  tCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAAt  <  2:383456/90‑1 (MQ=255)                                                                                   |                                                                         CACGTTCCACGATTTCTACCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCATCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAAT  >  NZ_CP009273/1854044‑1854198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑