Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,900,885	A→G	S145P (TCC→CCC)	rlmA ←	23S rRNA (guanine(745)‑N(1))‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,900,885	0	A	G	100.0%	42.9 / NA	14	S145P (TCC→CCC)	rlmA	23S rRNA (guanine(745)‑N(1))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/4);  total (10/4)

TGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGA  >  NZ_CP009273/1900823‑1900966                                                               |                                                                                  tGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                          <  1:371924/88‑1 (MQ=255) tGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                          >  2:371924/1‑88 (MQ=255)           tctGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTg                                              >  1:77443/1‑90 (MQ=255)                     acGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGAt                                    <  2:294440/90‑1 (MQ=255)                     acGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGAt                                    <  2:307184/90‑1 (MQ=255)                        gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGc                                 >  2:73531/1‑90 (MQ=255)                                  aCGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTcgccgc                       >  2:230090/1‑90 (MQ=255)                                  aCGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTcgccgc                       >  2:31117/1‑90 (MQ=255)                                              gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGAcaca                                                     >  1:188044/1‑48 (MQ=255)                                              gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGAcaca                                                     <  2:188044/48‑1 (MQ=255)                                              gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc           >  2:240059/1‑90 (MQ=255)                                              gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc           >  2:332788/1‑90 (MQ=255)                                              gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc           >  2:381419/1‑90 (MQ=255)                                                       ggTATCGGGAAACGGCAAGCGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGa  >  2:158598/1‑90 (MQ=255)                                                               |                                                                                  TGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGA  >  NZ_CP009273/1900823‑1900966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑