Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,057,664	T→C	G95G (GGA→GGG)	cobT ←	nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,057,664	0	T	C	90.0%	21.4 / ‑3.8	10	G95G (GGA→GGG)	cobT	nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (5/4);  total (5/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGAT  >  NZ_CP009273/2057589‑2057743                                                                            |                                                                                agcagTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTCCCACGGGTCATAtt                                                                   >  2:147562/1‑90 (MQ=255)      tATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTCCCACGGGTCATATTTTCAg                                                              <  1:149366/90‑1 (MQ=255)        tCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTCCCACGGGTCATATTTTCAGcc                                                            <  1:119005/90‑1 (MQ=255)        tCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTCCCACGGGTCATATTTTCAGcc                                                            <  2:55515/90‑1 (MQ=255)            tACCAACATCAATTCCGGGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACCCGCCGGTTG‑TCCACGGGTCATATTTTCAGCCTGTAt                                                       <  1:87425/90‑1 (MQ=255)               cAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTCCCACGGGTCATATTTTCAGCCTGTATgg                                                     >  2:339168/1‑90 (MQ=255)               cAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAG‑‑CACACACGCCGGTTGTCCCACGGGTCATATTTTCAGCCTGTATGGCt                                                   >  1:385052/1‑90 (MQ=255)                                    tAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTCCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGaaa                                >  2:292002/1‑90 (MQ=255)                                                gTTCTGCCAGCACACACACGCCGGTTGTCCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCtt                    <  1:98997/90‑1 (MQ=255)                                                                  cGCCGGTTGTCCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGAt  >  2:279402/1‑90 (MQ=255)                                                                            |                                                                                AGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGAT  >  NZ_CP009273/2057589‑2057743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑